A Secret Weapon For thr77
A Secret Weapon For thr77
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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has become noticed in several tumors, which ends up in chromosome instability, and therefore might lead to tumorigenesis. This protein was found to associate with, and be involved in, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a significant part in promoting mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, are actually explained. Two extra splice variants had been noted but specific nucleotide sequence information and facts just isn't however readily available.
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Blocking peptides are peptides that bind especially for the target antibody and block antibody binding. These peptide ordinarily has the epitope identified with the antibody.
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This information outlines further more probable optimization of mobile and immunoassay parameters to be certain the very best results are...
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This information outlines even more doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide no more bind to your epitope to the focus on protein. By comparing the staining in the blocked antibody vs . the antibody by itself, you can see which staining is distinct.
Goal Data Cyclin E belongs for the hugely conserved cyclin relatives, whose associates are characterized by a remarkable periodicity in protein abundance with the mobile cycle. Cyclins operate as regulators of CDK kinases. Different cyclins show distinct expression and degradation styles which lead to the temporal coordination of every mitotic function. Cyclin E forms a posh with and functions to be a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates on the G1-S phase boundary and is particularly degraded as cells development by means of S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by each antibodies...
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